FDBusiness.com

New ISO standard validates flow cytometry

 Breaking News
  • World’s Most Modern Snack Plant Begins Operations in Finland The world’s most modern dairy snack plant has commenced operation at Valio Riihimäki in Southern Finland. The snack plant is the largest single investment in Valio’s history. Construction of the new snack plant began in 2014 and took about three years. The area of the plant building is approximately 20,000 square metres. The plant is [...]...
  • FrieslandCampina Sells Fruit Juice Business The Dutch investment company Standard Investment is acquiring Riedel, the fruit juice business of FrieslandCampina, for an undisclosed sum. Based at Ede in the Netherlands, Riedel has a turnover of about €125 million and employs 200 people. Completion of the transaction is expected before the end of 2017. Earlier this year, FrieslandCampina announced that it would [...]...
  • LacPatrick is Cream of the Crop in UAE LacPatrick, the international dairy co-operative, has secured over £1 million of sales of its LP spray dried whole milk and skimmed milk powder in the United Arab Emirates (UAE), following participation at the Gulfood exhibition in February 2017. LacPatrick is one of Northern Ireland’s major manufacturing employers, employing over 300 staff across its Ballyrashane, Artigarvan [...]...
  • Tate & Lyle Launches CLARIA® Instant Functional Clean-Label Starches Tate & Lyle, a leading global provider of food ingredients and solutions, has announced a further expansion of its line of CLARIA® Functional Clean-Label Starches with the introduction of two new instant starch products: CLARIA® Instant 340 and 360. As claims such as ‘smooth’, ‘luscious’ and ‘velvet’ continue to grow, manufacturers are eager to develop textures [...]...
  • Reveal Impact – Ardagh Group to Launch a Two-Stage Thermochromic Solution Reveal Impact features combined temperature-sensitive inks with a twist. The new solution for aluminium cans uses two different thermochromic inks, which both boast the same colour when cooled down. However, once the can gets warmer again, one colour disappears quicker than the other, revealing a hidden message to the consumer. The revealed message surprises the [...]...
  • HKScan Opens New €80 Million Poultry Plant HKScan, the Nordic meat group, has just opened its new, state-of-the-art poultry unit at Rauma in Finland. Valued at over €80 million, the new facility is the largest investment in HKScan’s history. Indeed, the state-of-the-art Rauma unit ranks among the most significant investments in the history of the Finnish food industry. Jari Latvanen, president and chief [...]...

New ISO standard validates flow cytometry

February 02
11:42 2016

standardA new ISO standard (ISO 19344 (IDF 232)) describes a method for the quantification of lactic acid bacteria by flow cytometry in fermented products, starter cultures and probiotics used in dairy products. The publication is the result of the joint work of ISO and the International Dairy Federation (IDF).

Lactobacillus and bifidobacterium, present in yogurt and other dairy products, are well-known medical foods used as probiotics, or “friendly bacteria”, to maintain a healthy digestive tract. Flow cytometry, a cell-counting method for assessing the quality of cultures by determining the proportion of active cells, has met with a degree of scepticism. The new ISO standard is claimed to rubber-stamp the validity of this method, speeding up quality control and facilitating trade.

The standard provides quantification of lactic acid bacteria (LAB) and is important in assessing the quality of starter cultures, probiotics and fermented milk products. Examination of LAB in these products can be carried out following different methods, with plate count techniques being the most traditional and widely used. Newer techniques include flow cytometry, which is able to determine the proportion of active cells and/or total units.

“Advantages of the use of flow cytometry include low variation, reduction of testing time, differentiation between active and total cells and the possibility of high-throughput analysis,” said Dr. Sandra Casani, IDF/ISO Project Leader. “Furthermore, quantification of the fraction of active cells per total cells is a key feature of flow cytometry. This is of special relevance for certain applications, such as optimization of production processes and stability assessment during shelf life.”

This ISO/IDF project relied on the participation of producers and users of LAB as well as experts and users of flow cytometry from both industry and academia. This reflects the need and support for such a standard, which is crucial for obtaining general acceptance by the industry and for getting the recognition of this methodology by regulatory bodies.

“Joint standards such as this one are important to avoid duplication of work and ensure optimal and harmonized procedures in analysis and sampling of milk and milk products around the globe,” said Harrie van den Bijgaart, Chair of the ISO technical committee on milk and milk products (ISO/TC 34/SC 5) and Chair of the IDF Methods Standards Steering Group. “They also provide safeguards to the equivalence of testing results, whereas the availability of these well-respected joint standards also limits the required in-house validation efforts of the instrument users. The collaboration between IDF and ISO is key in achieving this.”

An international collaborative study of ISO 19344 (IDF 232) was conducted to determine precision figures, which validated that the method is fit for purpose.

About Author

admin

admin

Related Articles



Food & Drink Business Conference & Exhibition 2016

Upcoming Events

  • September 11, 2017drinktec
  • September 13, 2017FI Asia
  • September 19, 2017PROCESS EXPO 2017
  • September 22, 2017Global Summit on Food & Beverages
AEC v1.0.4

The Magazine

F&D Business Preferred Suppliers

Advertisements